ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY: A REVOLUTIONIZED LC TECHNIQUE

  • Shalini Bhardwaj
  • Vandana A.
  • Vijay B.
  • Manish K. Gupta
DOI https://doi.org/10.22270/ijdra.v2i3.146

Abstract

High Performance Liquid Chromatography (HPLC) is a major technique for qualitative and quantitative drug analysis. More than 90% of drugs prescribed in official pharmacopoeias are being analyzed HPLC. HPLC analyzes the drug content in a sample with high degree of accuracy and precision. Due to the stringent regulatory requirements the number of samples for drug content analysis has been increased significantly. Therefore, pharmaceutical industries need a fast, accurate and affordable method for drug content analysis. Here, Ultra Performance Liquid Chromatography (U-PLC) offers an advancement of HPLC which is based on the principal of use of stationary phase consisting of particles less than 2μm. By using smaller particles; speed and peak capacity can be extended to new limits and the sample can be analyzes in a shorter period of time. It provides good resolution even for congeneric compounds. The present review discusses the various aspects of UPLC in pharmaceutical analysis.

Keywords: Ultra Performance Liquid Chromatography (UPLC), HPLC, ACQUITY UPLC.

Downloads

Download data is not yet available.

References

1. Van Deemter JJ, Zuiderweg FJ, Klinkenberg A. Longitudinal diffusion and resistance to mass transfer as causes of nonideality in chromatography. Chem. Eng. Sci. 1956; 5:271.
2. Cheng, YF, Lu, Z, Neue U. Ultrafast liquid chromatography/ultraviolet and liquid chromato- graphy/tandem mass spectrometric analysis. Rapid Commun. Mass Spectrom. 2001; 15(2): 141.
3. Tiller PR, Romanyshyn LA, Neue UD. Fast LC/MS in the analysis of small molecules Anal. Bioanal. Chem. 2003 Nov; 377(5): 788-802.
4. Neue UD, Mazzeo JR. A theoretical study of the optimization of gradients at elevated temperature J. Sep. Sci. 2001; 24(12): 921-229.
5. Swartz ME. Ultra Performance Liquid Chromatography (UPLC): An Introduction, Separation Science Re-Defined. LCGC Supplement; 2005 May .p. 8.
6. Jerkovich AD, Mellors JS, Jorgenson JW. The Use of Micron-Sized Particles in Ultrahigh-Pressure Liquid Chromatography. LCGC. 2003; 21(7):600-10.
7. MacNair JE, Lewis KC Jorgenson JW. Ultrahigh-pressure reversed-phase liquid chromatography in packed capillary columns, Anal. Chem. 1997; 69(6): 983–9.
8. Swartz ME. Ultra Performance Liquid Chromatography (UPLC): An Introduction, Separation Science Re-Defined, LCGC Supplement; 2005 May. p. 10.
9. MacNair JE, Patel KD, Jorgenson JW. Anal. Chem. 71; 1999.p.700–8
10. Wu N, Lippert JA, Lee ML. Practical aspects of ultrahigh pressure capillary liquid chromatography. J. Chromatogr. A, 2001 March; 911(1): 1-12.
11. Swartz ME. Ultra Performance Liquid Chromatography (UPLC): An Introduction, Separation Science Re-Defined, LCGC Supplement; 2005 May. p. 12.
12. Swartz ME. Ultra Performance Liquid Chromatography (UPLC): An Introduction, Separation Science Re-Defined, LCGC Supplement; 2005 May. p. 11.
13. Lippert JA, Xin B, Wu N, Lee ML. J. Microcolumn Sep. 1997; 11: 631–43.
14. Swartz ME. Ultra Performance Liquid Chromatography (UPLC): An Introduction, Separation Science Re-Defined, LCGC Supplement; 2005 May. p. 13.
15. Jerkovitch AD, Mellors JS, and Jorgenson JW, LCGC North America. 2003;21:7.
16. Swartz M., LCGC 23(1), 46–53 (2005).
17. Broske AD, et al. Agilent Technologies application note. 5988-9251EN; 2004.
18. Goodwin L, White SA, Spooner N. Evaluation of ultra-performance liquid chromatography in the bioanalysis of small molecule drug candidates in plasma. J. Chromatogr. Sci.2007; 45(6): 298–304.
Statistics
491 Views | 511 Downloads
How to Cite
1.
Bhardwaj S, A. V, B. V, Gupta MK. ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY: A REVOLUTIONIZED LC TECHNIQUE. Int J Drug Reg Affairs [Internet]. 2018Feb.13 [cited 2024Sep.11];2(3):83-7. Available from: https://ijdra.com/index.php/journal/article/view/146
Issue
Vol 2 No 3 (2014): VOLUME 2, ISSUE 3, 2014
Section
Review
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

The International Journal of Drug Regulatory affairs require a formal written transfer of copyright from the author(s) for each article published. We therefore ask you to complete and return this form, retaining a copy for your records. Your cooperation is essential and appreciated. Any delay will result in a delay in publication.

I/we have read and agree with the terms and conditions stated Page 2 of this agreement and I/we hereby confirm the transfer of all copyrights in and relating to the above-named manuscript, in all forms and media, now or hereafter known, to the International Journal of Drug Regulatory affairs, effective from the date stated below. I/we acknowledge that the IJDRA is relying on this agreement in publishing the above-named manuscript. However, this agreement will be null and void if the manuscript is not published in the IJDRA.

Download link for COPYRIGHT FORM