ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY: A REVOLUTIONIZED LC TECHNIQUE

  • Shalini Bhardwaj
  • Vandana A.
  • Vijay B.
  • Manish K. Gupta

Abstract

High Performance Liquid Chromatography (HPLC) is a major technique for qualitative and quantitative drug analysis. More than 90% of drugs prescribed in official pharmacopoeias are being analyzed HPLC. HPLC analyzes the drug content in a sample with high degree of accuracy and precision. Due to the stringent regulatory requirements the number of samples for drug content analysis has been increased significantly. Therefore, pharmaceutical industries need a fast, accurate and affordable method for drug content analysis. Here, Ultra Performance Liquid Chromatography (U-PLC) offers an advancement of HPLC which is based on the principal of use of stationary phase consisting of particles less than 2μm. By using smaller particles; speed and peak capacity can be extended to new limits and the sample can be analyzes in a shorter period of time. It provides good resolution even for congeneric compounds. The present review discusses the various aspects of UPLC in pharmaceutical analysis.

Keywords: Ultra Performance Liquid Chromatography (UPLC), HPLC, ACQUITY UPLC.

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How to Cite
Bhardwaj, S., V. A., V. B., and M. K. Gupta. “ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY: A REVOLUTIONIZED LC TECHNIQUE”. International Journal of Drug Regulatory Affairs, Vol. 2, no. 3, Feb. 2018, pp. 83-87, doi:10.22270/ijdra.v2i3.146.