• Md Hedaitullah
  • Ramanpreet Walia
  • Iqbal Khalid
  • Balwan Singh
  • Asif Hussain


Pyrazoline derivatives, being used as potential medicinal agents, possess many important pharmacological activities and therefore they are useful materials in drug research. A series of these derivatives HT- 1 to HT- 6 containing  5-4-(chlorophenyl)-1,3-diphenyl- 4,5-dihydro-1H-pyrazole derivatives were synthesized, structures were confirmed using melting point, IR, NMR and MS and evaluated for their antibacterial activity using disc diffusion method at concentration 2mg/ml. The reference used was Amikacin and mostly, all synthesized compounds exhibited a significant antibacterial activity against Staphylococcus aureus (MTCC No. 96) as Gram positive bacteria, Staphylococcus epidermidis(MTCC No. 435) as Gram positive and Escherichia coli (MTCC No. 739) as Gram negative bacteria. It was observed that HT-1 and HT-2 possess good antibacterial potential against S. aureus, HT- 5 against S.epidermidis and HT-1 and HT-4against E. coli.

Keywords: Pyrazoline, Disc diffusion method, Amikacin.


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1. Taylor EC, Patel H, Kumar H, et al. Synthesis of pyrazolo 3,4-d pyrimidine analogues of the potent agent N-4-2-2-amino-4 3H-oxo-7H-pyrrolo 2,3-d pyrimidin-5-yl ethylbenzoyl-L-glutamic acid (LY231514). Tetrahedron. 1992; 48:8089-8100.
2. Lombardino JG, Otterness IG, et al. Novel immunosuppressive agents. Potent immunological activity of some bensothiopyrano [4,3-c]pyrazol-3-one. J Med Chem. 1981; 24: 830-34.
3. Holla BS, Akberali PM, Shivananda MK, et al. Studies on arylfuran derivatives: Part X. Synthesis and antibacterial properties of arylfuryl-Δ2-pyrazolines.Il Farmaco. 2000; 55:256-63.
4. Ali MA, Yar MS, Kumar M, Pandian GS, et al. Synthesis and antitubercular activity of substituted novel pyrazoline derivatives. Nat Prod Res. 2007 Jun; 21(7):575-9.
5. Bansal E, Srivatsava VK, Kumar A , et al. Synthesis and anti-inflammatory activity of 1-acetyl-5-substituted aryl – 3 - (beta-aminonaphthyl) – 2 -pyrazolines and beta-(substituted aminoethyl) amidonaphthalenes. Eur J Med Chem. 2001; 36: 81-92.
6. Manna F, Chimenti F, Fioravanti R, Bolasco A, Seecci D, Chimenti P, Ferlini C, Scambia G, et al. Synthesis of some pyrazole derivatives and preliminary investigation of their affinity binding to P-glycoprotein. Bioorg Med Chem Lett. 2005; 15: 4632-35.
7. Ahn JH, Kim HM, Jung SH, Kang SK , Kim KR, Rhee SD, Yang SD, Cheon HG, Kim SS, et al. Synthesis and DP-IV inhibition of cyano-pyrazoline derivatives as potent anti-diabetic agents. Bioorg Med Chem Lett. 2004; 14: 4461- 65.
8. Prasad YR, Rao AL, Prasoona L, Murali K, Kumar PR, et al. Synthesis and antidepressant activity of some 1,3,5-triphenyl-2-pyrazolines and 3-(2H- hydroxynaphthalen-1-yl)-1,5-diphneyl- 2-pyrazolines. Bioorg Med Chem Lett. 2005; 15: 5030-34.
9. Mulder R, Wellinga K, Van Daalen JJ, et al. A new class of insecticides. Naturwissenschaften. 1975; 62: 531-32.
10. Cruickshank R, Duguid JP, Marion BP, Swain RHA, et al. The practice of medicinal microbiology, 12th ed., vol. II. London: Churchil Livingstone; 1975.p. 196-202.
11. Collins AH (Ed.). Microbiological Methods, 2nd ed. London: Butterworth; 1976.p. 24.
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How to Cite
Hedaitullah, M., R. Walia, I. Khalid, B. Singh, and A. Hussain. “PYRAZOLINE SYNTHESIS THROUGH A CHALCONE INTERMEDIATE”. International Journal of Drug Regulatory Affairs, Vol. 2, no. 4, Feb. 2018, pp. 59-62, doi:10.22270/ijdra.v2i4.153.